Methods : Highlights
: Methods Articles
Editorial: Byte-ing off more than you can chewWith access to high-throughput technologies, researchers struggle to store their raw data. Many just give up. Nature Methods, vol. 5 #7, pp577-577 |
Research Highlights: Fish fingers on the menuZebrafish researchers rejoice! Reverse genetics is now on the menu, thanks to zinc-finger nucleases. Nature Methods, vol. 5 #7, pp579-579 |
Research Highlights: Living dropletsTiny droplets of water in oil can serve as miniature culture vessels for living single cells and multicellular organisms. Nature Methods, vol. 5 #7, pp580-581 |
Research Highlights: Chemical biology: New electrophilic probes slide inRecently discovered electrophilic probes open the door to activity-based protein profiling (ABPP) studies of a broader range of proteins. Nature Methods, vol. 5 #7, pp580-581 |
Research Highlights: Live-cell map questA high-resolution interactome map that describes how proteins interact in living yeast cells is an invaluable reference for the research community. Nature Methods, vol. 5 #7, pp582-582 |
News and Views: The beginning of the end for microarrays?Two complementary approaches, both using next-generation sequencing, have successfully tackled the scale and the complexity of mammalian transcriptomes, at once revealing unprecedented detail and allowing better quantification. Nature Methods, vol. 5 #7, pp585-587 |
News and Views: Hunting hidden transcriptsStrategies for the comprehensive identification of transcript isoforms produced from specific genomic loci make use of and expand existing tools and resources. Nature Methods, vol. 5 #7, pp587-589 |
News and Views: Microfluidics: streamlining discovery in worm biologyAdvances in the application of microfluidics technology to biological assays using the model organism Caenorhabditis elegans help to automate otherwise time-consuming experiments. Nature Methods, vol. 5 #7, pp589-590 |
Brief Communication: Isoform discovery by targeted cloning, 'deep-well' pooling and parallel sequencingThe complete set of coding sequences, including all splice isoforms, is not known for any metazoan organism. Combination of a normalized pooling scheme and a new assembly algorithm with 454 sequencing yields a methodological pipeline for isoform discovery. The validated pipeline may now be applied genome-wide. Nature Methods, vol. 5 #7, pp597-600 |
Brief Communication: Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivoConventional techniques for generating transgenic mice are quite costly, require substantial resources and necessitate killing the mouse. In contrast, in vivo electroporation of repopulating spermatogonial cells in the mouse testis can produce male mice for siring multiple distinctive transgenic founders for over a year. Nature Methods, vol. 5 #7, pp601-603 |
Brief Communication: Lifeact: a versatile marker to visualize F-actinCurrent approaches for live imaging of cellular actin dynamics have several drawbacks. Now the use of Lifeact, a 17-aa actin-binding peptide from yeast that is not present in higher eukaryotes, allows imaging of actin dynamics in live mammalian cells without disruption of function and without competition with endogenous binding proteins. Nature Methods, vol. 5 #7, pp605-607 |
Brief Communication: In vitro whole-organ imaging: 4D quantification of growing mouse limb budsA combination of improved in vitro embryo culture and optical projection tomography allows development of the mouse limb bud to be monitored over time. Developmental changes seen in vitro are benchmarked against in vivo development, and tissue movements are quantitatively described. Nature Methods, vol. 5 #7, pp609-612 |