: Methods Articles
Research Highlights: Phosphorylation and the cell cycleTwo groups used quantitative mass spectrometry to look at changes in protein phosphorylation across the cell cycle. Nature Methods, vol. 5 #10, pp858-859 |
News and Views: Classical genetics goes high-techA combination of automated screening and next-generation sequencing makes it possible to identify Caenorhabditis elegans mutants at unprecedented speed and scale. Nature Methods, vol. 5 #10, pp863-864 |
Research Highlights: A surrogate scaffold testedResearchers tested an alternate antibody scaffold, creating so-called Surrobodies. Nature Methods, vol. 5 #10, pp861-861 |
Research Highlights: AntibodypediaA web portal to share antibody validation data. Nature Methods, vol. 5 #10, pp860-860 |
Research Highlights: Microbes right on targetResearchers use a targeted metagenomic approach to functionally characterize complex microbial communities. Nature Methods, vol. 5 #10, pp857-857 |
Research Highlights: New twists on photoswitchable proteinsFluorescent proteins with new photoswitching properties allow multilabel imaging at a single detection wavelength and dual-color superresolution microscopy. Nature Methods, vol. 5 #10, pp858-859 |
Editorial: Elective affinitiesA feasibility study for the systematic generation of affinity reagents to human proteins provides an opportunity to test the merits of recombinant affinity reagents. Nature Methods, vol. 5 #10, pp851-851 |
Brief Communication: Automated screening for mutants affecting dopaminergic-neuron specification in C. elegansAn automated sorting method using the COPAS Biosort machine allows the isolation of mutant C. elegans displaying differences in GFP expression in small numbers of cells. Compared to manual methods this increases the efficiency of the phenotypic selection step in cell-fate screens. Nature Methods, vol. 5 #10, pp869-872 |
Brief Communication: Building consensus spectral libraries for peptide identification in proteomicsSpectral searching, based on matching experimental peptide spectra to reference spectral libraries, is gaining interest as an alternative to traditional sequence-database searching in mass spectrometry–based proteomics. A software tool, SpectraST, now allows users to build their own high-quality spectral libraries from raw data. Nature Methods, vol. 5 #10, pp873-875 |
Brief Communication: Imaging individual mRNA molecules using multiple singly labeled probesA strategy using 48 or more singly labeled fluorescent oligonucleotide probes targeted to individual mRNA molecules allows the simultaneous localization and quantification of three mRNA species in fixed cells. mRNA visualization in whole animals and other organisms is also demonstrated. Nature Methods, vol. 5 #10, pp877-879 |
Brief Communication: Caenorhabditis elegans mutant allele identification by whole-genome sequencingIdentifying the molecular lesions in mutants isolated in forward genetic screens can be a laborious process. A proof-of-principle study in Caenorhabditis elegans now shows that this can be achieved rapidly by whole-genome deep sequencing. Nature Methods, vol. 5 #10, pp865-867 |