シロイヌナズナでの重複受精の際に生じるカルシウムスパイクのライブ画像化

Ca²⁺ waves and oscillation are key signalling elements during the fertilization process of animals, and are involved, for example, in egg activation. In the unique double fertilization process in flowering plants, both the egg cell and the neighbouring central cell fuse with a sperm cell each. Here we succeeded in imaging cytosolic Ca²⁺ in these two cells, and in the two synergid cells that accompany the gametes during semi-in vivo double fertilization. Following pollen tube discharge and plasmogamy, the egg and central cells displayed transient Ca²⁺ spikes, but not oscillations. Only the events in the egg cell correlated with the plasmogamy. In contrast, the synergid cells displayed Ca²⁺ oscillations on pollen tube arrival. The two synergid cells showed distinct Ca²⁺ dynamics depending on their respective roles in tube reception. These Ca²⁺ dynamics in the female gametophyte seem to represent highly specific signatures that coordinate successful double fertilization in the flowering plants.